Research Abstract


Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells

2015年2月10日 Scientific Reports 5 : 8344 doi: 10.1038/srep08344


Taku Tanaka, Tadashi Yokoi, Fuminobu Tamalu, Shu-Ichi Watanabe, Sachiko Nishina & Noriyuki Azuma

Corresponding Author

東 範行
国立成育医療研究センター 感覚器・形態外科部 眼科

We generated self-induced retinal ganglion cells (RGCs) with functional axons from human induced pluripotent stem cells. After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted in differentiation of RGCs at a rate of nearly 90% as indicated by a marginal subregion of an extruded clump of cells, suggesting the formation of an optic vesicle. Axons extended radially from the margin of the clump. Induced RGCs expressed specific markers, such as Brn3b and Math5, as assessed using by quantitative PCR and immunohistochemistry. The long, prominent axons contained neurofilaments and tau and exhibited anterograde axonal transport and sodium-dependent action potentials. The ability to generate RGCs with functional axons uniformly and at a high rate may contribute to both basic and clinical science, including embryology, neurology, pathognomy, and treatment of various optic nerve diseases that threaten vision.