Research Abstract


Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

2014年6月23日 Scientific Reports 4 : 5400 doi: 10.1038/srep05400


佐久間 哲史1, 西川 綾美1, 久米 悟士1, 茶山 一彰2 & 山本 卓1

  1. 広島大学大学院 理学研究科 数理分子生命理学専攻
  2. 広島大学大学院 医歯薬保健学研究院
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette. We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. Our system provides an efficient targeting strategy for multiplex genome/epigenome editing, simultaneous activation/repression of multiple genes, and beyond.