Research Abstract



Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform

2013年10月25日 Nature Communications 4 : 2684 doi: 10.1038/ncomms3684


ステファン・ディーリング1, 王 丹1, 金 致源2, 近藤 美欧1, 陳 勇1, 北川 進1,2, 亀井 謙一郎1 & 古川 修平1

  1. 京都大学 物質-細胞統合システム拠点
  2. 京都大学工学研究科 合成・生物化学専攻
Functional cellular substrates for localized cell stimulation by small molecules provide an opportunity to control and monitor cell signalling networks chemically in time and space. However, despite improvements in the controlled delivery of bioactive compounds, the precise localization of gaseous biomolecules at the single-cell level remains challenging. Here we target nitric oxide, a crucial signalling molecule with site-specific and concentration-dependent activities, and we report a synthetic strategy for developing spatiotemporally controllable nitric oxide-releasing platforms based on photoactive porous coordination polymers. By organizing molecules with poor reactivity into polymer structures, we observe increased photoreactivity and adjustable release using light irradiation. We embed photoactive polymer crystals in a biocompatible matrix and achieve precisely controlled nitric oxide delivery at the cellular level via localized two-photon laser activation. The biological relevance of the exogenous nitric oxide produced by this strategy is evidenced by an intracellular change in calcium concentration, mediated by nitric oxide-responsive plasma membrane channel proteins.