Research Abstract


Functional salivary gland regeneration by transplantation of a bioengineered organ germ

2013年10月1日 Nature Communications 4 : 2498 doi: 10.1038/ncomms3498


小川 美帆1,2, 大島 正充2, 今村 彩3, 関根 由利恵3, 石田 研太郎2, 山下 健太郎3, 中島 啓4, 平山 雅敏5, 立川 哲彦6 & 辻 孝1,2,3

  1. 株式会社 オーガンテクノロジーズ
  2. 東京理科大学 総合研究機構
  3. 東京理科大学院 基礎工学研究科 生物工学専攻
  4. 東京歯科大学 大学院歯学研究科 臨床検査病理学講座
  5. 慶應義塾大学 大学院医学研究科 外科系眼科学
  6. 昭和大学 歯学研究科 口腔病理学講座
Salivary gland hypofunction, also known as xerostomia, occurs as a result of radiation therapy for head cancer, Sjögren’s syndrome or aging, and can cause a variety of critical oral health issues, including dental decay, bacterial infection, mastication dysfunction, swallowing dysfunction and reduced quality of life. Here we demonstrate the full functional regeneration of a salivary gland that reproduces the morphogenesis induced by reciprocal epithelial and mesenchymal interactions through the orthotopic transplantation of a bioengineered salivary gland germ as a regenerative organ replacement therapy. The bioengineered germ develops into a mature gland through acinar formations with a myoepithelium and innervation. The bioengineered submandibular gland produces saliva in response to the administration of pilocarpine and gustatory stimulation by citrate, protects against oral bacterial infection and restores normal swallowing in a salivary gland-defective mouse model. This study thus provides a proof-of-concept for bioengineered salivary gland regeneration as a potential treatment of xerostomia.