Research Abstract


In vivo assessment of cancerous tumors using boron doped diamond microelectrode

2012年11月29日 Scientific Reports 2 : 901 doi: 10.1038/srep00901


Stephane Fierro1, 吉川 桃子2, 永野 修2, 吉見 建二3, 佐谷 秀行2, 4 & 栄長 泰明1, 4

  1. 慶應義塾大学 理工学部 化学科
  2. 慶應義塾大学 医学部 先端医科学研究所 遺伝子制御研究部門
  3. 順天堂大学 医学部 生理学第一講座
  4. 独立行政法人 科学技術振興機構 戦略的創造研究推進事業(JST-CREST)
The in vitro and in vivo electrochemical detection of the reduced form of glutathione (L-γ-glutamyl-L-cysteinyl-glycine, GSH) using boron doped diamond (BDD) microelectrode for potential application in the assessment of cancerous tumors is presented. Accurate calibration curve for the determination of GSH could be obtained by the in vitro electrochemical measurements. Additionally, it was shown that it was possible to separate the detection of GSH from the oxidized form of glutathione (GSSG) using chronoamperometry measurements. In vivo GSH detection measurements have been performed in human cancer cells inoculated in immunodeficient mice. These measurements have shown that the difference of GSH level between cancerous and normal tissues can be detected. Moreover, GSH detection measurements carried out before and after X-ray irradiation have proved that it is possible to assess in vivo the decrease in GSH concentration in the tumor after a specific treatment.