Research Abstract


A chemical genomic study identifying diversity in cell migration signaling in cancer cells

2012年11月8日 Scientific Reports 2 : 823 doi: 10.1038/srep00823


間木 重行1, 田代 悦1 & 井本 正哉1

  1. 慶應義塾大学理工学部 生命情報学科 ケミカルバイオロジー研究室
The aim of this study was to analyze the diversity and consistency of regulatory signaling in cancer cell migration, using a chemical genomic approach. The effects of 34 small molecular compounds were assessed quantitatively by wound healing assay in ten types of migrating cells. Hierarchical clustering was performed on the subsequent migration inhibition profile of the compounds and cancer cell types. The result was that hierarchical clustering accurately classified the compounds according to their targets. Furthermore, the cancer cells tested in this study were classified into three clusters, and the compounds were grouped into four clusters. An inhibitor of JNK suppressed all types of cell migration; however, inhibitors of ROCK, GSK-3 and p38MAPK only inhibited the migration of a subset of cell lines. Thus, our analytical system could easily distinguish between the common and cell type-specific signals responsible for cell migration.