Research Abstract


Single molecule detection from a large-scale SERS-active Au79Ag21 substrate

2011年10月10日 Scientific Reports 1 : 112 doi: 10.1038/srep00112


Hongwen Liu1, Ling Zhang1, Xingyou Lang1, 山口 佳則3, 岩崎 裕3, 井上 康志2, Qikun Xue1,4 & Mingwei Chen1,5

  1. 東北大学原子分子材料科学高等研究機構
  2. 大阪大学大学院生命機能研究科
  3. 大阪大学フォトニクス先端融合研究拠点
  4. 清華大学(中国)
  5. 上海交通大学(中国)
Detecting and identifying single molecules are the ultimate goal of analytic sensitivity. Single molecule detection by surface-enhanced Raman scattering (SM-SERS) depends predominantly on SERS-active metal substrates that are usually colloidal silver fractal clusters. However, the high chemical reactivity of silver and the low reproducibility of its complicated synthesis with fractal clusters have been serious obstacles to practical applications of SERS, particularly for probing single biomolecules in extensive physiological environments. Here we report a large-scale, free standing and chemically stable SERS substrate for both resonant and nonresonant single molecule detection. Our robust substrate is made from wrinkled nanoporous Au79Ag21 films that contain a high number of electromagnetic ‘‘hot spots’’ with a local SERS enhancement larger than 109. This biocompatible gold-based SERS substrate with superior reproducibility, excellent chemical stability and facile synthesis promises to be an ideal candidate for a wide range of applications in life science and environment protection.