Research Abstract


Cytokine-based high log-scale expansion of functional human dendritic cells from cord-blood CD34-positive cells

2011年11月29日 Scientific Reports 1 : 174 doi: 10.1038/srep00174


原田 結1,2,3, 岡田(仲西)八栄3, 上田 泰次2,5, 辻谷 俊一4, 齊藤 智2,3, 藤(小川)照美2, 飯田 章博5, 長谷川 護5, 市川 智彦1 & 米満 吉和2,3

  1. 千葉大学大学院医学研究院 泌尿器科学
  2. 千葉大学大学院医学研究院 遺伝子治療学
  3. 九州大学大学院薬学研究院 革新的バイオ医薬創成学
  4. 九州大学大学院医学研究院 消化器・総合外科
  5. DNAVEC株式会社
Dendritic cells (DCs) play a crucial role in maintaining the immune system. Though DC-based cancer immunotherapy has been suggested as a potential treatment for various kinds of malignancies, its clinical efficacies are still insufficient in many human trials. Issues that limit the clinical efficacy of DC-based immunotherapy, as well as the difficulty of the industrial production of DCs, are largely due to the limited number of autologous DCs available from each patient. We here established a possible breakthrough, a simple cytokine-based culture method to expand the log-scale order of functional human DCs. Floating cultivation of cord-blood CD34+ cells under an optimized cytokine cocktail led these progenitor cells to stable log-scale proliferation and to DC differentiation. The expanded DCs had typical features of conventional myeloid DCs in vitro. Therefore, the concept of DC expansion should contribute significantly to the progress of DC immunotherapy.