Research Abstract


Cell-Sized confinement in microspheres accelerates the reaction of gene expression

2012年2月22日 Scientific Reports 2 : 283 doi: 10.1038/srep00283


加藤 絢子1, 柳澤 実穂2, 佐藤 祐子1, 藤原 慶3 & 吉川 研一1

  1. 京都大学大学院 理学研究科
  2. 九州大学大学院 理学研究院
  3. 東北大学大学院 工学研究科
Cell-sized water-in-oil droplet covered by a lipid layer was used to understand how lipid membranes affect biochemical systems in living cells. Here, we report a remarkable acceleration of gene expression in a cell-sized water-in-oil droplet entrapping a cell-free translation system to synthesize GFP (green fluorescent protein). The production rate of GFP (VGFP) in each droplet remained almost constant at least for on the order of a day, which implies 0th-order reaction kinetics. Interestingly, VGFP was inversely proportional to radius of droplets (R) when R is under 50 μm, and VGFP in droplets with R ∼ 10 μm was more than 10 times higher than that in the bulk. The acceleration rates of GFP production in cell-sized droplets strongly depended on the lipid types. These results demonstrate that the membrane surface has the significant effect to facilitate protein production, especially when the scale of confinement is on the order of cell-size.