Research press release


Nature Medicine

Ultrasensitive detection of prions


異常な立体構造をもち病原性を示すプリオンタンパク質(PrPSc)を試験管内で増幅する技術が開発されたことにより、CJDの新しい診断法ができる可能性が出てきた。新 竜一郎(長崎大学)たちが開発したPrPSc検出法はリアルタイムQUIC(RT-QUIC:real-time quaking-induced conversion)法と呼ばれ、CJD患者の脳抽出液中のわずか1フェムトグラム(10-15グラム)のPrPScを短時間で検出できる。


An ultrasensitive method to detect human prions — the agent that causes Creutzfeldt-Jakob disease (CJD) — is reported in an article published online this week in Nature Medicine.

The development of technologies for the in vitro amplification of the pathogenic conformation of the prion protein — PrPSc — has generated the potential for new diagnostic assay for CJD. Ryuichiro Atarashi and his colleagues developed a PrPSc detection assay, called real-time quaking-induced conversion (RT-QUIC), which allows the fast detection of as little as one femtogram — 10-15 grams — of PrPSc in extracts from brains of people with CJD.

Crucially, the technique allowed the authors to detect prions with high sensitivity and specificity in cerebrospinal fluid samples from patients, highlighting the potential of RT-QUIC for the early detection of suspected CJD cases.

doi: 10.1038/nm.2294


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