Hsa-miR-520d-5p promotes survival in human dermal fibroblasts exposed to a lethal dose of UV irradiation

2016年11月24日 npj Aging 2, Article number:16029 (2016) doi:10.1038/npjamd.2016.29




Yoshitaka Ishihara, Satoshi Tsuno, Bingqiong Ping, Taichiro Ashizaki, Masahiro Nakashima, Keigo Miura, Yugo Miura, Taro Yamashita, Junichi Hasegawa and Norimasa Miura

Anti-aging: miR-520d-5p salvages DNA-fragmented cells from lethal nuclear stress
As hsa-miR-520d-5p has an innovating effect of reversion of undifferentiated cancer cells to benign or normal status via a stemness-mediated process, we attempted to examine its reprogramming effect on differentiated normal cells (dermal fibroblasts). We found that miR-520d-5p has a restoration effect on fibroblasts exposed to lethal ultraviolet B irradiation and that it induced these cells to the mesenchymal status with CD105 expression. The therapeutic effect indicates that miR-520d-5p is deeply involved in DNA repair (non-canonical pathway against nuclear stress), and it may function as the main regulator in the response mechanism to fragmented DNA and severely damaged nucleic acids. Therefore, this study may pave the way to elucidating a new mechanism because miRNA may play a role in the biogenesis of cells that are getting lethal due to cell damage or aging.


npj Aging and Mechanisms of Disease EISSN: 2056-3973(online)