Volume 552 Number 7685

Editorials

News

News Features

Nature's 10 p.315

Ten people who mattered this year.

doi: 10.1038/d41586-017-07763-y

News & Views

Energy from thin air p.336

Advanced genomic-analysis techniques now suggest that microbial communities in cold, nutrient-poor Antarctic soils can acquire their energy from the oxidation of trace gases, rather than by photosynthesis.

doi: 10.1038/d41586-017-07579-w

The origins of memory T cells p.337

Memory T cells protect against previously encountered pathogens, but their origins are unclear. Two studies track DNA modifications over time and find that these cells arise from effector T cells.

doi: 10.1038/d41586-017-08280-8

Martian water stored underground p.339

Why did Mars lose so much of its surface water, whereas Earth retained its? Models of the evolution of minerals on the two planets suggest one explanation: the Martian water was drawn into the planetary interior.

doi: 10.1038/d41586-017-08670-y

Tumour lymph vessels boost immunotherapy p.340

A high level of expression of the growth-factor protein VEGF-C is associated with tumours that have extensive lymph vessels and poor prognosis. It emerges that such tumours are highly susceptible to immunotherapy.

doi: 10.1038/d41586-017-08669-5

Specks of insight into Alzheimer’s disease p.342

Inflammatory cues trigger microglial cells to release the protein ASC. It emerges that specks of ASC promote a hallmark of Alzheimer’s disease in the brains of mice — aggregation and deposition of amyloid-β protein.

doi: 10.1038/d41586-017-08668-6

Articles

Microglia-derived ASC specks crossseed amyloid-β in Alzheimer’s disease p.355

The spreading of pathology within and between brain areas is a hallmark of neurodegenerative disorders. In patients with Alzheimer’s disease, deposition of amyloid-β is accompanied by activation of the innate immune system and involves inflammasome-dependent formation of ASC specks in microglia. ASC specks released by microglia bind rapidly to amyloid-β and increase the formation of amyloid-β oligomers and aggregates, acting as an inflammation-driven cross-seed for amyloid-β pathology. Here we show that intrahippocampal injection of ASC specks resulted in spreading of amyloid-β pathology in transgenic double-mutant APPSwePSEN1dE9 mice. By contrast, homogenates from brains of APPSwePSEN1dE9 mice failed to induce seeding and spreading of amyloid-β pathology in ASC-deficient APPSwePSEN1dE9 mice. Moreover, co-application of an anti-ASC antibody blocked the increase in amyloid-β pathology in APPSwePSEN1dE9 mice. These findings support the concept that inflammasome activation is connected to seeding and spreading of amyloid-β pathology in patients with Alzheimer’s disease.

doi: 10.1038/nature25158

Letters

Cryo-EM structures of the TMEM16A calcium-activated chloride channel p.426

Calcium-activated chloride channels (CaCCs) encoded by TMEM16A control neuronal signalling, smooth muscle contraction, airway and exocrine gland secretion, and rhythmic movements of the gastrointestinal system. To understand how CaCCs mediate and control anion permeation to fulfil these physiological functions, knowledge of the mammalian TMEM16A structure and identification of its pore-lining residues are essential. TMEM16A forms a dimer with two pores. Previous CaCC structural analyses have relied on homology modelling of a homologue (nhTMEM16) from the fungus Nectria haematococca that functions primarily as a lipid scramblase, as well as subnanometre-resolution electron cryo-microscopy. Here we present de novo atomic structures of the transmembrane domains of mouse TMEM16A in nanodiscs and in lauryl maltose neopentyl glycol as determined by single-particle electron cryo-microscopy. These structures reveal the ion permeation pore and represent different functional states. The structure in lauryl maltose neopentyl glycol has one Ca2+ ion resolved within each monomer with a constricted pore; this is likely to correspond to a closed state, because a CaCC with a single Ca2+ occupancy requires membrane depolarization in order to open (C.J.P. et al., manuscript submitted). The structure in nanodiscs has two Ca2+ ions per monomer and its pore is in a closed conformation; this probably reflects channel rundown, which is the gradual loss of channel activity that follows prolonged CaCC activation in 1 mM Ca2+. Our mutagenesis and electrophysiological studies, prompted by analyses of the structures, identified ten residues distributed along the pore that interact with permeant anions and affect anion selectivity, as well as seven pore-lining residues that cluster near pore constrictions and regulate channel gating. Together, these results clarify the basis of CaCC anion conduction.

doi: 10.1038/nature25024