Peanut leaf proteome
doi:10.1038/nindia.2010.41 Published online 31 March 2010
Researchers have, for the first time, conducted a comprehensive proteome analysis of peanut leaf outlining its functions related to growth, development and responses to stresses. The study promises to improve the understanding of plant genetics and metabolism and assist in the selection and breeding programmes geared towards crop improvement.
The group obtained umpteen candidate proteins from systems biology mediated protein-protein interaction studies on Arachis hypogea or peanut, one of the most important sources of plant protein.
The study will also serve as the basis for further investigations of peanut physiology such as detection of expressed changes due to biotic and abiotic stresses and plant development. The leaf proteome map will lead to development of protein markers for cultivar identification at seedling stage of the plant, the authors say.
In peanut, water stress significantly lowers plant resistance to Aspergillus flavus infection leading to aflatoxin contamination of peanut seed. One of the strategies adopted to decrease the risk of contamination is to identify and develop drought-tolerant peanut genotypes through molecular breeding.
The current selection of genotypes requires molecular characterisation of available populations. Peanut genome database has several EST cDNAs which can be used to analyse gene expression. Analysis of proteins is a direct approach to define function of their associated genes, the authors say.
Proteome analysis linked to genome sequence information is critical for functional genomics. However, available protein expression data is extremely inadequate. The finding advances knowledge in functional genomics of peanut.
The authors of this work are from: Florida A&M University & Mississippi State University, USA and Bioclues.org, India.
- Katam, R. et al. Analysis of Peanut Leaf Proteome. J. Proteome Res. doi: 10.1021/pr901009n (2010)