: Methods Articles
Editorial: Find them earlyGetting young researchers into independent positions that encourage risk-taking would benefit science but requires more than targeted individual research grants. Nature Methods, vol. 7 #3, pp163-163 |
Research Highlights: New tools to enlighten brain functionThe pioneers of the first generation of widely used optogenetic tools now describe new variants that open further possibilities for studying neural function. Nature Methods, vol. 7 #3, pp173-173 |
Research Highlights: Immortal strands versus silent sistersA method to differentially label each sister chromatid in a cell makes it possible to elucidate segregation patterns after mitosis and should help to pinpoint the mechanism behind nonrandom segregation in certain cell types. Nature Methods, vol. 7 #3, pp174-175 |
Research Highlights: A genome in timeAddition of next-generation sequencing to an assay of replication timing enables high-resolution genome-scale analyses of multiple cell types. Nature Methods, vol. 7 #3, pp174-175 |
Research Highlights: A global map of gene functionResearchers map functional interactions for 75% of the yeast genome using synthetic genetic array methodology. Nature Methods, vol. 7 #3, pp176-176 |
Research Highlights: Smart delivery with nanofactoriesResearchers engineer biological 'nanofactories' that can trigger a quorum sensing response in bacteria. Nature Methods, vol. 7 #3, pp178-178 |
Research Highlights: Reliably bright miceMice with inducible reporter genes allow systemic profiling of gene expression throughout the brain. Nature Methods, vol. 7 #3, pp180-180 |
News and Views: Waltz, an exciting new move in amyloid predictionA new amyloid-prediction tool, Waltz, offers advantages over previous amyloid-prediction tools for distinguishing 'true' amyloids from amorphous aggregates. Nature Methods, vol. 7 #3, pp187-188 |
News and Views: aMAZe-ing tools for mosaic analysis in zebrafishMosaic analysis in zebrafish (MAZe) allows lineage tracing and analysis of mosaic animals. Nature Methods, vol. 7 #3, pp188-190 |
News and Views: The electronic crystal ball: predicting cell fate from time-lapse dataProspective isolation of defined cell types is a crucial prerequisite for their molecular analysis, but the heterogeneity of populations yielded by current protocols obscures relevant information. New studies now use additional features from time-resolved imaging data for live prospective identification of cells with defined future behavior. Nature Methods, vol. 7 #3, pp190-191 |
Brief Communication: A nonviral minicircle vector for deriving human iPS cellsSimple minicircle vectors carrying four reprogramming factors induce pluripotency in adult human adipose stem cells and in neonatal fibroblasts without integration into the genome. Nature Methods, vol. 7 #3, pp197-199 |
Brief Communication: In-cell recordings by extracellular microelectrodesConventional extracellular electrode recordings are generally limited to monitoring action potentials. But use of extracellular gold microelectrodes with microspines that are engulfed by a neuron generates efficient electrical coupling and allows detection of both action potentials and subthreshold synaptic potentials with a signal-to-noise ratio similar to that of conventional intracellular recordings. Nature Methods, vol. 7 #3, pp200-202 |
Brief Communication: Detecting the conformation of individual proteins in live cellsSingle-molecule fluorescence resonance energy transfer (smFRET) is applied in live cells and reveals the conformational changes of individual SNARE proteins upon entering a SNARE complex. Nature Methods, vol. 7 #3, pp203-205 |
Brief Communication: Organelle-specific, rapid induction of molecular activities and membrane tetheringChemically inducible dimerization probes selectively target proteins to the surface of specific organelles or tether organelles to each other, thus allowing precise spatiotemporal analysis of signaling events. Nature Methods, vol. 7 #3, pp206-208 |
Brief Communication: Temporal pixel multiplexing for simultaneous high-speed, high-resolution imagingBy subdividing a charge-coupled device (CCD) array into subgroups using a digital micromirror device and offsetting exposure times, temporal pixel multiplexing allows simultaneous high-speed and high-resolution imaging using a single CCD. This imaging modality allows 250 Hz microscopic imaging of fast cellular responses with a 10-Hz 1.3 megapixel camera Nature Methods, vol. 7 #3, pp209-211 |
