Research Highlights

doi:10.1038/nindia.2013.65 Published online 21 May 2013

Fungal polysaccharide boosts immune system

Researchers have isolated a glucan (a polysaccharide) from the fruit bodies of Ramaria botrytis, a fungus that grows in hilly forests1. The polysaccharide is capable of activating white blood cells that boost immunity during various diseases.

The fungal fruit bodies are attractive as food. Recent studies have shown that they harbour polysaccharides with antitumour potential. However, no previous study had explored the therapeutic potential of the fruit bodies of R. botrytis.

To identify polysaccharides with therapeutic potential, the researchers boiled small pieces of R. botrytis fruit bodies and then cooled and filtered the extracts. They took the filtrate and processed it to yield water-soluble crude polysaccharide. Using chemical and spectroscopic analyses, they identified the polysaccharide as glucan.

The researchers then carried out experiments to probe glucan's potential to boost the immune system. For this, they used mouse macrophage, an immune cell that surrounds and destroys pathogens and cells in mouse spleen and thymus. The spleen is rich in splenocytes — cells that generate various white blood cells such as T and B cells, which stimulate immune responses; the thymus contains thymocytes that generate T cells.

At various concentrations, the glucan was found to enhance the production of splenocytes and thymocytes, suggesting that the glucan can boost the immune response. The study has shown that the glucan is nontoxic to healthy cells. Other studies have shown that glucans do not kill cancer cells; rather they exhibit antitumour activity by stimulating the host's immune system without injuring normal cells.

"Fungal glucan can be used as an immunostimulating agent for fighting different immunosuppressive diseases including cancers," says Syed S. Islam, a co-author of the study.

The authors of this work are from: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, Department of Chemistry, Darjeeling Government College, Darjeeling, and Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal, India.


References

  1. Bhanja, S. K. et al. Structural studies of an immunoenhancing glucan of an ectomycorrhizal fungus Ramaria botrytis. Carbohydr. Res. 374, 59-66 (2013) | Article | PubMed |