Methods : Highlights
: Methods Articles
Editorial: BiocloudsUnderstanding how cloud computing can serve the scientific community is a research question in its own right and one that researchers?with the help of funders?should address. Nature Methods, vol. 7 #7, pp481-481 |
Research Highlights: Advent of synthetic lifeResearchers synthesized the first functional synthetic bacterial genome; repeating this feat with different bacteria will require further methodological development. Nature Methods, vol. 7 #7, pp487-487 |
Research Highlights: A toolset for the proficient geneticistNew strategies expand the genetic toolkit for transgene expression, lineage tracing and mosaic analysis of gene function in flies and mammalian cells. Nature Methods, vol. 7 #7, pp488-489 |
Research Highlights: Where do you come from?Live-cell time-lapse imaging of somatic cells undergoing reprogramming raises interesting questions about the mechanism of the process. Nature Methods, vol. 7 #7, pp488-489 |
Research Highlights: Robotics on the nanoscaleNanoscale robots can be programmed to walk a DNA origami track. Nature Methods, vol. 7 #7, pp490-490 |
Research Highlights: Searching for mismatches in a vast genomic landscapeRaw data of millions of sequences used to assemble the reference genomes of ten organisms are analyzed in search of mismatches indicative of editing events. Findings include candidate sites for in vivo DNA and RNA editing, and a common sequencing error. Nature Methods, vol. 7 #7, pp492-492 |
Research Highlights: Faster, sharper electron microscopyA technique combining laser and electron pulses is used to achieve nanometer and femtosecond resolution in biological imaging. Nature Methods, vol. 7 #7, pp494-494 |
News and Views: Illuminating eukaryotic transcription start sitesSimplified methods to map transcription start sites allow the exploration of transcription-initiation landscapes in rare cell types. Nature Methods, vol. 7 #7, pp501-503 |
News and Views: Watching the fly brain in actionNew technical feats make it possible to monitor the activity of identified neurons in awake behaving Drosophila melanogaster. Nature Methods, vol. 7 #7, pp505-506 |
News and Views: The inside view on plant growthWith a combination of microscopic and computational methods, the lineage of cells produced by divisions in the meristems of growing plants can now be tracked over time. Nature Methods, vol. 7 #7, pp506-507 |
Brief Communication: Proteome-wide analysis of protein carboxy termini: C terminomicsA method to analyze the sequence of C-terminal peptides using a combination of a specific enrichment approach and mass spectrometry is described, allowing the study of C-terminal proteolytic processing on a global scale. Also in this issue, Van Damme et al. describe a related method for simultaneous C- and N-terminal peptide analysis. Nature Methods, vol. 7 #7, pp508-511 |
Brief Communication: Complementary positional proteomics for screening substrates of endo- and exoproteasesA method to simultaneously analyze C- and N-terminal peptides using a combination of strong cation exchange, diagonal chromatography and mass spectrometry is described, allowing the screening of protease substrates on a global scale. Also in this issue, Schilling et al. describe a related method for analyzing the sequence of C-terminal peptides. Nature Methods, vol. 7 #7, pp512-515 |
Brief Communication: Simultaneous intracellular chloride and pH measurements using a GFP-based sensorA genetically encoded ratiometric biosensor not based on fluorescence resonance energy transfer (FRET), ClopHensor, allows concurrent measurement of intracellular pH and chloride by providing an internal control for pH-dependent fluorescence changes. Measurements of chloride levels in acidic large dense core vesicles showed high concentrations of chloride. Nature Methods, vol. 7 #7, pp516-518 |
Brief Communication: Estimating prion concentration in fluids and tissues by quantitative PMCAThe misfolded form of the prion protein, PrPSc, can be quantified in a variety of tissues and fluids using a quantitative version of the popular protein misfolding cyclic amplification (PMCA) assay. Nature Methods, vol. 7 #7, pp519-520 |
