Methods : Highlights
: Methods Articles
Editorial: The call of the human proteomeA Human Proteome Project has been proposed. To ensure that such a project will have a transformative impact on biology, its scope should be determined by wide and open discussion. Nature Methods, vol. 7 #9, pp661-661 |
Research Highlights: Unnatural designResearchers designed an enzyme to carry out the Diels-Alder reaction, an activity not found in nature. Nature Methods, vol. 7 #9, pp671-671 |
Research Highlights: Single molecules meet systems biologyApplication of single-molecule methods to assay genome-wide mRNA and protein levels in single bacteria provides a systems-level view of their relationship. Nature Methods, vol. 7 #9, pp672-673 |
Research Highlights: Learning from historyThe strength of selection that acts on a growing population of cells and the presence of a stochastic switching mechanism may be inferred from lineage data. Nature Methods, vol. 7 #9, pp672-673 |
Research Highlights: When bacteria experience a tremorA trackable multiplex recombineering method in Escherichia coli allows for the identification of genes that affect a selectable trait. Nature Methods, vol. 7 #9, pp674-674 |
Research Highlights: Rapid allosteric activationEngineered kinases remain off in cells until turned on by small molecules. Nature Methods, vol. 7 #9, pp676-676 |
Research Highlights: Melting trapped DNASingle DNA molecules stretched in a nanochannel display melting patterns characteristic of their sequence. Nature Methods, vol. 7 #9, pp678-678 |
News and Views: C. elegans selectThe technical toolkit for Caenorhabditis elegans expands to include experimental selection using antibiotic resistance genes. Nature Methods, vol. 7 #9, pp693-695 |
News and Views: Stem cells feel the differenceArrays of microposts of different heights generate substrates with different flexibility, on which cells can be grown. Nature Methods, vol. 7 #9, pp695-697 |
News and Views: Oxygen maps in the brainOxygen concentrations in the rodent brain are revealed at a microscopic scale by measuring the lifetime of two photon?excited phosphorescence. Nature Methods, vol. 7 #9, pp697-699 |
Brief Communication: Live-cell super-resolution imaging with trimethoprim conjugatesUse of a trimethoprim chemical tag allows super-resolution live-cell microscopy by stochastic single molecule?based localization imaging of the dynamics of genetically tagged histone H2B in cell nuclei. Nature Methods, vol. 7 #9, pp717-719 |
Brief Communication: An antibiotic selection marker for nematode transgenesisA gene conferring neomycin resistance can be used for antibiotic selection in C. elegans and C. briggsae. This will permit easy maintenance of transgenic lines and facilitate single-copy insertion of transgenes. Also in this issue, a related paper reports nematode selection using puromycin. Nature Methods, vol. 7 #9, pp721-723 |
Brief Communication: Rapid selection of transgenic C. elegans using antibiotic resistanceA gene conferring puromycin resistance can be used for antibiotic selection in C. elegans and C. briggsae. This will permit easy maintenance of transgenic lines and facilitate single-copy insertion of transgenes. Also in this issue, a related paper reports nematode selection using neomycin. Nature Methods, vol. 7 #9, pp725-727 |
Brief Communication: Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-NanoEngineering of the Ca2+-sensing domain in existing yellow Cameleon Ca2+ indicators is used to create indicators with a range of increased Ca2+ affinities capable of detecting subtle changes in intracellular Ca2+ at low resting levels. Nature Methods, vol. 7 #9, pp729-732 |
Brief Communication: Mechanical regulation of cell function with geometrically modulated elastomeric substratesMicropost arrays can be used to modulate substrate rigidity independently of other substrate properties, permitting the study of the effects of rigidity on cell function. Nature Methods, vol. 7 #9, pp733-736 |
Brief Communication: Automated imaging with ScanLag reveals previously undetectable bacterial growth phenotypesA simple automated system to simultaneously measure growth rate and lag time of large numbers of bacteria permits the identification of subsets in a heterogenous population. Nature Methods, vol. 7 #9, pp737-739 |
